Subject who has provided informed consent and is dressed in sleeping attireĪ well ventilated room for electrode applicationĪbrading cream, such as NuPrep, SkinPure, or Lemon Prep from a supplier such as Integra NeuroSupplies or MVAP.Įlectrode conductive paste, such as Ten20 (MVAP and Integra Neurosupplies)Įlectrode conductive gel, such as Signa gel (MVAP and Integra Neurosupplies)ĮEG electrodes – gold or silver/silver chloride10 mm diameter cup electrodes with a 48” wire lead with safety connector (Grass, MVAP and Integra Neurosupplies) Materials (Rather than metric units, materials are listed in commonly available dimensions.) Note: Research involving human subjects should adhere to all local and national regulations that ensure protection of human subjects. This protocol assumes that the subjects have been appropriately screened and are suitable for study. The subject's sleep schedule in the days prior to recording can greatly affect the sleep and EEG on the recording night, as can various illegal, prescription, and over the counter drugs. The EEG recorded on a study night depends strongly on the subject's history prior to recording. The following protocol describes calibration and EEG recording on an ambulatory recorder, but a clinical recorder in a laboratory could substitute for an ambulatory recorder. Computer digitization and recording have replaced paper recording, and handheld ambulatory recorders now can replace whole racks of amplifiers. EEG amplifiers and recording instruments have changed greatly in the past 20 years. The protocol provides instruction on EEG recorder calibration, electrode application, EEG recording, and spectral analysis of the EEG.
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Although the focus is on use for sleep research, the methods can be adapted for other fields of neuroscience investigation. This unit presents methods for recording and analyzing the human electroencephalogram (EEG). EEG Recording and Analysis for Sleep Research